Persia Jothy

4 Questions 3 Answers 0 Followers

Questions related from Persia Jothy

Protocol for lipoprotein staining using Sudan black B in SDS-PAGE?

I want to quantify the amount of lipid present in lipoprotein by SDS-PAGE analysis. What is the protocol to stain the SDS gel for lipids?

07 August 2017 8,184 3 View

Can we use HCL instead of Perchloric acid in Phospholipid estimation?

Many Phospholipid estimation protocol is having perchloric acid for acidic digestion.. Can i use HCL instead of Perchloric acid?

21 August 2016 8,567 1 View

Is it necessary to perform TLC before HPLC in Carotenoid purification?

I am extracting carotenoid pigments from animal tissues like ovary, hepatopancreas etc. of prawn using solvent system Methanol and Dichloromethane. After extraction, can I directly go for HPLC for...

18 August 2016 6,910 3 View

How to get lipids alone without the interference of carotenoids?

After extracting lipids from gonadal tissue of crustacean by Folch method, colored lipid fraction is obtained. The lipid fraction is mixed with carotenoid pigments. Is there any method to get...

01 January 1970 3,526 2 View