Where can I download an MRI dataset of the human pelvis?

I am looking for a good/high-quality MRI dataset of the human pelvic region. Do you know where I can find this? Thanks!

13 October 2020 7,738 5 View

Has anyone received negative RFU values for one of your replicates in qPCR?

We are running qPCR with SYBRGreen to amplify an inserted 12S ribosomal DNA region of a plasmid. When we ran this amplification for the first time (in duplicate), one replicate looked fine while...

03 April 2018 4,926 4 View

Interpolation of 3D wind data (for every lon,lat,time) based on drifter positions and time (different matrix dimensions)?

Hello everyone, I am working with some meteorological data from SKIRON which I converted from grib to netcdf in order to make the necessary calculations and formatting for interpolation. I want to...

17 January 2018 6,415 3 View

What are the best procedures and techniques of teaching STEM education in the twenty first century?

I am interested in searching for the best procedures and techniques of teaching STEM to motivate and sustain students' interest in STEM particularly chemistry. p

05 September 2017 9,622 3 View

Is there a problem with using a large sample size in your data analysis after you have determined a small sample size before the study?

29 May 2017 6,595 10 View

Labelling proteins for interaction studies

What labels do people use most frequently for studying protein interactions in vitro and why? And what wavelengths will be required if I use these labels? The reason I ask is we are developing an...

09 May 2017 8,689 2 View

Does injection of 18F-FDG induce an endogenous insulin response similar to normal glucose?

29 September 2016 5,189 2 View

What is the minimum soil sample size (in g) that should be used when wet sieving to examine mycorrhizal fungi spore abundance and/or diversity?

10 July 2016 7,749 11 View

Urgent: does this analysis of a repeated measure manova make sense?

background: 100 students were given an unrelated text and a related text each. they then had to answer questions on each text, say how interested they were in each text, and then evaluate how much...

30 May 2016 6,467 3 View

How do I generate circular ssDNA from linear dsDNA?

starting material: linear dsDNA wanted product: circular ssDNA

04 January 2016 1,664 5 View

Can anyone advise me on how to amplify V4 region of fecal DNA of dss-induced colitic mice for microbiota profiling?

Animals were fed DSS via drinking water and fecal samples were collected and DNA was isolated using Qiagen stool DNA isolation kit. The V4 region of extracted DNA was amplified with specific...

20 December 2020 1,739 3 View

Comparison of Pitting Potential of different Stainless steels?

Dear Corrosion Experts, Is there any research paper comparing various stainless steels ASS, DSS, MSS at different pH, Cl- ion concentration and Temperature? please share if someone has...

27 January 2020 5,300 4 View

I am working to automate the HEC RAs i am stuck with .DSS creation file . what is the proces to create time series data to dss.... in automode . ?

I am working to automate the HEC RAs i am stuck with .DSS creation file . what is the proces to create time series data to dss.... in auto-mode . i want to create real time rainfall data to .dss file

11 December 2018 7,891 0 View

How to make mouse colon single cell suspension and preserve these cells for flow cytometry?

hi, guys. I am treat dss-induced mouse colitis model with anti-inflammation herbs now and I need to check the M1/M2 polarization in the inflammation site of the colon. May I know how to make mouse...

08 September 2018 410 0 View

Do you know a paper in which DSS treated mice exhibit normal weight loss but increased inflammation ?

I have seen a paper like that but I cannot seem to find it again. I am interested also interested in papers using other models of chemically-induced colitis on mice.

07 August 2018 7,196 0 View

What 's the dose of dextran sodium sulfate (DSS) on inducing colon cancer model?

Hi, Generally, people establish the rat/mice colon cancer model by treating the animal with 1,2-Dimethylhydrazine and 1-5% DSS in drinking bottle, but I want to ask, can we administer DSS orally...

07 August 2018 5,208 0 View

Does anyone know about chronic DSS models where transgenic mice have reduced weight lost in DSS phase but delayed weight recovery in water phase?

Hello everyone, I just started to use the DSS model on my transgenic mice which showed better resistance to the DSS treatment (2%) but then reduced recovery in the water phase compared to WT mice....

05 June 2018 2,408 0 View

Why I get low level of mature M1 ratio in spleen of DSS-induced mouse colitis model?

Hi, guys, I did DSS-induced colitis model recently to find whether my targeted herb has the anti-inflammatory or immunomodulatory function in inflammation mouse model. However, I find the ratio of...

05 June 2018 1,914 0 View

Which one is better for treating experimental colitis, sulfasalazine or 5-amino salicylic acid?

I want to induce colitis by DSS and then use phytochemical to check it's effect in disease amelioration. In this process i want to use standard drug to check the performance of my phytochemical.

04 May 2018 2,013 2 View

Which mice is better for DSS induced experimental colitis, BALB/c or C57BL/6?

I want to induce experimental colitis by oral administration of 5% DSS. Now as I see there are many papers suggesting different things about experimental mice for DSS induce colitis model, I am...

04 May 2018 5,248 5 View