Why should i administer DSS through drinking water for colitis model in mice?
What's the difference of DSS administration between drinking water and p.o?
If I administer DSS per orally, should i restrict additional water after administration?
As shown in the figures, there are several abnormal charge/discharge curves.Each of them may have different problems. I am curious why these situations appeared differently?There are a lot of...
24 July 2024 6,930 1 View
I have a protein complex in which some dimers easily dissociate. What methods can I use to stabilize these dimers within the complex without altering the overall structure of the complex? Will...
16 July 2024 2,757 2 View
Hello, Does anybody have idea about any software or database that can give information about the possible phospho sites located in a protein? I am trying PhosIDP but its not working or loading...
15 July 2024 1,940 3 View
Hi, may I know I want to fuse my protein with affinity tag for protein purification in Expi293, how can I determine and visualise how does a N or C terminal tag affect my proteins (structure,...
24 June 2024 8,622 6 View
We have measured the ZFC/FC curve under 100Oe, there is splitting happens and there is another turning point happens to around 25K, why it is increased again with decreasing temperature?
11 June 2024 9,603 1 View
I need assistance. I submitted a paper to the DEXA2024 conference, and it has been accepted. However, I am unable to attend the conference held in Italy. Now, I am looking for a partner whose...
12 May 2024 8,431 0 View
I have a mat that have 3 data about grid_X、grid_Y and Mb,i want save the tif in ArcGIS use the mat. mat file in the attachment.
09 May 2024 1,541 1 View
I'm eager to hear your professional views about patient psychology!
04 May 2024 4,497 2 View
hey, I am designing gRNA target site to check the endogenous expression of protein in zebrafish. For this I am using chop-chop site. But most of the sites are in exonic region. I couldn't find...
13 April 2024 3,757 3 View
Hello everyone. Could someone advise on the likelihood of high-purity SWCNT-COOH undergoing oxidation when stored in a refrigerator? I suspect that my SWCNT-COOH may have been oxidized due to the...
31 March 2024 2,524 0 View
Approximate concentrations are require in compared with the WHO permissible limts
11 August 2024 2,723 1 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I am developing a predictive model for a water supply network that involves 20 influencing points. However, I only have historical data for 10 out of these 20 points. I would like to know how to...
10 August 2024 4,005 2 View
How do interactions between the biosphere, the carbon cycle, and the water cycle impact global warming and interaction between the atmosphere and the hydrosphere?
09 August 2024 3,291 2 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
One can try to generalize the Vandermonde determinant in the following direction: Let $A$ be any symmetric $n$-order square matrix. Consider its powers' diagonal elements $(A^k)_{ii}$ and...
08 August 2024 6,690 1 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View