Why do i have an decreasing number of pores in R10.4.1 flow cell (nanopore)?

19 December 2023 0 9K Report

The number of pores in the R.10.4.1 flow cell decreased significantly from +/- 1400 to 291 after nanopore sequencing with only 24 samples multiplexing. I used the SQK-RPB114-24 kit for processing the 24 samples as one library. Would anyone recommend anything about the protocol or to change something about it? Does anyone have about the same experience and what did you do to make it in some way better?

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