Naomi Thorne

3 Questions 3 Answers 0 Followers

Questions related from Naomi Thorne

Protocol for DSS cross-linking in adherent cells?

Hi, I am looking to try a cross-linking protocol to assess alpha-synuclein species in my adherent cells. I have DSS for this and have found several papers with a semi-descriptive method but I...

04 September 2023 3,705 0 View

Tyrosine hydroxylase by Western blot - doublet or not?

Hi, I have run a couple of Western blots for tyrosine hydroxylase in mammalian SH-SY5Y cells and have noticed 2 distinct bands rather than just one, as is shown in most of the literature. Some...

31 May 2023 8,037 2 View

Methods for detecting aggregated alpha-synuclein species in mammalian cells?

I have two SH-SY5Y cell lines that stably overexpress alpha-synuclein; one the WT protein and one the A53T mutant form. I am interested to see whether I have any accumulations or aggregates of...

24 May 2023 2,437 5 View