How to crosslink my antibody to protein G in Dynabeand protein G IP experiment with DSS?

13 March 2023 0 4K Report

As mentioned in the title, I can't find the protocol of using DSS to do crosslinking dynabead protein G with antibody on the Internet. Can anyone who has experience give me some tips, thank you very much.

The DSS and dynabead protein G I use are both products from Thermo (#H51771.ME and #10007D)

Also, I found that DSS is very hygroscopic, can anyone with experience suggest if I should aliquot or make a stock solution when I first open it?

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