Where can I download an MRI dataset of the human pelvis?

I am looking for a good/high-quality MRI dataset of the human pelvic region. Do you know where I can find this? Thanks!

13 October 2020 7,738 5 View

Has anyone received negative RFU values for one of your replicates in qPCR?

We are running qPCR with SYBRGreen to amplify an inserted 12S ribosomal DNA region of a plasmid. When we ran this amplification for the first time (in duplicate), one replicate looked fine while...

03 April 2018 4,926 4 View

Interpolation of 3D wind data (for every lon,lat,time) based on drifter positions and time (different matrix dimensions)?

Hello everyone, I am working with some meteorological data from SKIRON which I converted from grib to netcdf in order to make the necessary calculations and formatting for interpolation. I want to...

17 January 2018 6,415 3 View

What are the best procedures and techniques of teaching STEM education in the twenty first century?

I am interested in searching for the best procedures and techniques of teaching STEM to motivate and sustain students' interest in STEM particularly chemistry. p

05 September 2017 9,622 3 View

Is there a problem with using a large sample size in your data analysis after you have determined a small sample size before the study?

29 May 2017 6,595 10 View

Does injection of 18F-FDG induce an endogenous insulin response similar to normal glucose?

29 September 2016 5,189 2 View

What is the minimum soil sample size (in g) that should be used when wet sieving to examine mycorrhizal fungi spore abundance and/or diversity?

10 July 2016 7,749 11 View

Urgent: does this analysis of a repeated measure manova make sense?

background: 100 students were given an unrelated text and a related text each. they then had to answer questions on each text, say how interested they were in each text, and then evaluate how much...

30 May 2016 6,467 3 View

How can we confirm transfection of a WT gene into a cell line?

We are transfecting mutations and matched WT genes. We'll be using antibiotic selection and have generated kill curves to determine the optimal concentration of the selectable marker in the cells...

19 May 2015 4,820 8 View

What other factors can affect failure of hydrogels to form?

06 April 2015 7,411 4 View

How can you bring two structures together to create an adhesive bond in LAMMPS?

I have created an Ice 1h crystalline structure and an Aluminium substrate structure and equilibrated both at 250K. Now I need to bring them together in a way that an adhesive bond is created....

01 March 2021 3,325 2 View

Necessary tube lenght to cool Natural gas in a dry-ice cooling bath?

I have a tank which contains natural gas at 250 Bar and 293 Kelvin. I want to cool the gas in a dry-ice cooling bath to about 195 Kelvin. The gas will flow in a copper-tube through the bath with a...

27 February 2021 8,128 1 View

How to raise temperature in small increments during equilibration in LAMMPS?

All, I am trying to equilibrate an Ice 1h crystalline structure to 250K in LAMMPS. When I tried to do this at 250K directly, the structure lost it's crystalline shape and became disordered post...

14 February 2021 5,747 2 View

Should the Ice 1h crystalline structure be lost during equilibration process in LAMMPS?

I have created an Ice 1h structure and am equilibrating it 100K in NVT ensemble in LAMMPS for a timestep of 0.25fs in 3000 steps. The potential used is TIP4P/Ice. Although the simulation runs fine...

26 January 2021 9,603 11 View

How to remove a sapphire disk from LR white resin embedding ?

Hello, We tried to do CLEM experiment on sapphire disk including cryo fixation (Leica ICE) and then cryo substitution using LR white resin. Once the resin is polymerized, we can't remove the...

13 January 2021 5,223 3 View

What can happen in the world if large scale melting of continental and alpine glaciers occurs?

Several environmental experts have warned about the global warming and its effects on different ecosystems. It may also cause large scale melting of continental and alpine glaciers. If this...

23 December 2020 4,630 3 View

Cryo-EM Vitrobot settings for large lipid structures. How can I reduce ice thicknes?

Dear all, I have an issue figuring out good plunging conditions for large lipid structures: I have carbon coated C-Flat 2/2 400mesh copper grids with large (elongated) lipid structures on it....

13 December 2020 6,696 2 View

Why the success rate of SD rat modelling is so low?

Why the success rate of SD rat modelling is so low, ask for experience sharing The specific operation is as follows: SD male rats, weighing about 290g, mfb site injection. The concentration of...

09 November 2020 6,323 2 View

How do I make nano-sized particles of Polyethylene?

I am attempting to make particles in the range of 50-500 nm from Low density polyethylene. My current protocol is to dissolve 0.1 g of LDPE in xylene at 100 C, then drip the resultant solution...

08 November 2020 7,764 1 View

Nano Ice films sublimate due to low dose beam and annealing under vacuum? which one is more likely dominant?

An ice film sublimates under a severe vacuum pressure and a low does of electron beam. when the sample is in-situ annealed the sublimation rate significantly induced. In an isobaric process,...

06 November 2020 7,576 2 View