Which analysis in gromacs can determine the validity of a defined topology and show the best topology among different topologies?

Hi , I am a beginner in gromacs. I have made some topology files for graphene sheet with different bonded and non-bonded parameters I found in the literature. Now, how can I choose the best...

08 May 2020 7,509 3 View

Peptide lost during reverse phase HPLC?

Hello; I've dissolved my synthetic peptide in pure acetic acid and tried to purify it with reverse phase HPLC. I got weak peaks during analysis, however I injected at least 5 mg of my peptide!...

24 March 2019 3,467 4 View

How to calculate Kd with flowcytometric analysis?

Hello; I want to calculate Kd value for a FITC-conjugated peptide which specifically binds to a cell receptor. I've studied some protocols, but still confused! Does anyone have any experience or...

02 December 2018 3,385 2 View

What is the optimal concentration of G418 for selection of HEK293-T cells?

I've transfected HEK293-T cells with pCI as a mammalian expression vector containing neomycin gene as a selectable marker. I need to know the most efficient concentration of G418 for cell...

25 April 2015 1,154 4 View

How can I remove the free HC-LC from my antibody?

I have reduced the IgG4 to half antibody using TCEP. I see some (20%) free heavy and light chain (SDS-page), but they are still bound to each other (noncovalently). I see only one peak during...

09 February 2015 1,686 3 View

Sudden stop in recombinant protein expression in BL21?

I have cloned a 25KD fragment in pet32a and I got recombinant expression in BL21. The yield of expression was very good, but suddenly it stopped expressing!I tried transforming new BL21 bacteria...

08 February 2015 3,947 1 View

Is there any simple method to evaluate immune responses in immunized animals?

I have immunized rabbits and mice with DNA and I got no antibody answer in ELISA test! I need to know is there any other method to consider the presence of immune response?! I mean I want to know...

07 February 2015 2,730 5 View

Is Tolerance possible in DNA vaccination?

I'm working on polyclonal antibody production through genetic immunization in rabbits and after 5 month of immunization I got nothing! I need to know is there a problem with Tolerance induction...

01 January 2015 1,772 4 View

What is the exact sequence or structure difference between dimeric and tetrameric form of pyruvate kinase M2 in tumor cells?

Articles have mentioned they used antibodies to specifically detect the dimeric form but there's no sequence difference between dimer/tetramer as far as I know!

13 August 2013 1,940 1 View

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02 March 2021 3,060 3 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Has anyone experienced changes in growth for cell culture when purchasing media from different companies?

I recently switched the company where we buy our DMEM/F12 and i have noticed a signficant change in growth for my human olfactory neurosphere cell lines

24 February 2021 2,268 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

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22 February 2021 9,932 11 View

Is there a suitable way to confine cell attachment area inside a multi-well plate?

Are there any types of cylindrical cell culture inserts that are designed to confine cell seeding area? Say, if I'm using a 12 well plate (3.5 cm^2/well), but only want to seed cells in the middle...

22 February 2021 7,127 4 View