Raheleh Tooyserkani

10 Questions 12 Answers 0 Followers

Questions related from Raheleh Tooyserkani

How to stabilize polyclonal antibody in solution (after protein G column affinity chromatography)?

Hello; I perform antibody purification using protein G column, eluting the antibody in PB. Then I prepare several antibody aliquots and store them in -20 C. I use this polyclonal antibody as...

13 September 2021 7,974 3 View

Peptide lost during reverse phase HPLC?

Hello; I've dissolved my synthetic peptide in pure acetic acid and tried to purify it with reverse phase HPLC. I got weak peaks during analysis, however I injected at least 5 mg of my peptide!...

25 March 2019 3,560 4 View

How to correlate MFI and positive cell percentage in flow cytometry data?

Hello; I've treated my cells with IFN-gamma to induce PDL-1 expression. Then, I investigate whether a synthetic peptide specific to PDL-1 could bind PDL-1, through flow cytometry. To show peptide...

28 January 2019 9,919 6 View

How to calculate Kd with flowcytometric analysis?

Hello; I want to calculate Kd value for a FITC-conjugated peptide which specifically binds to a cell receptor. I've studied some protocols, but still confused! Does anyone have any experience or...

03 December 2018 3,488 2 View

Protocol for covalent conjugation of protein G to magnetic beads

Hello; I need a practical protocol to conjugate protein G to magnetic beads. Does anyone have any idea about that? Thanks

18 June 2017 4,866 4 View

What is the optimal concentration of G418 for selection of HEK293-T cells?

I've transfected HEK293-T cells with pCI as a mammalian expression vector containing neomycin gene as a selectable marker. I need to know the most efficient concentration of G418 for cell...

26 April 2015 1,295 4 View

Sudden stop in recombinant protein expression in BL21?

I have cloned a 25KD fragment in pet32a and I got recombinant expression in BL21. The yield of expression was very good, but suddenly it stopped expressing!I tried transforming new BL21 bacteria...

09 February 2015 4,029 1 View

Is there any simple method to evaluate immune responses in immunized animals?

I have immunized rabbits and mice with DNA and I got no antibody answer in ELISA test! I need to know is there any other method to consider the presence of immune response?! I mean I want to know...

08 February 2015 2,811 5 View

Is Tolerance possible in DNA vaccination?

I'm working on polyclonal antibody production through genetic immunization in rabbits and after 5 month of immunization I got nothing! I need to know is there a problem with Tolerance induction...

02 January 2015 1,880 4 View

What is the exact sequence or structure difference between dimeric and tetrameric form of pyruvate kinase M2 in tumor cells?

Articles have mentioned they used antibodies to specifically detect the dimeric form but there's no sequence difference between dimer/tetramer as far as I know!

14 August 2013 2,041 1 View