Why does a Nested PCR protocol amplify samples and not amplify positive control?

I have this problem. Today I used a new positive control of an isolated NC-1 strain in cell culture and the problem persists. I performed a serial dilution test to test the sensitivity of the...

08 December 2020 6,252 2 View

How to avoid the formation of traces and dimers in DNA gel electrophoresis?

07 December 2020 3,052 3 View

Is the pipetting order of the positive and negative controls in the PCR important to avoid in a possible contamination?

I have been watching bands form on my negative controls. I have already changed all reagents, optimized the hygiene of materials and the environment and increased care in pipetting.

07 December 2020 9,090 3 View

Best methodology to calculate food footprint in a university campus through students inquiring?

Hello, Considering sustainability and environment negative impacts of animal based products, I would like to calculate food footprint in a Portuguese university campus through students...

05 December 2020 9,098 3 View

How to standardize and obtain a good PCR reaction with samples with a low and variable amount of DNA?

I work with the detection of N. caninum DNA in milk samples in milk samples. The DNA concentrations in my samples range between 2-90 ng / ul. I would like to establish and standardize a final...

01 December 2020 8,792 3 View

How to quantify relative amounts of a substance with immunostainings through confocal microscopy when samples have high autofluorescence?

I am working with plant tissues, buds specifically, fixed in glutaraldehyde and embedded in LR White resin. I am working with the primary antibodies LM19 and LM20 targeting pectins. I am using a...

21 September 2020 5,213 6 View

What tools do you use for development and training on a remote machine?

Hi, I currently use vscode with the remote add-on for this and a ssh session with tmux. With this setup I can edit files, debug and run scripts remotly. What I use: Python, C++, ROS, Gazebo,...

14 May 2020 3,760 3 View

COVID-19, Human behaviour and wildlife impacts?

COVID-19 is causing a radical changes in Human behaviour around the world. Mandatory or voluntary isolation for months, suspension of the active management, etc.These changes will have positive...

24 March 2020 4,039 32 View

There are any component of gram + bacteria cell wall that can be used as a positive control?

Hello, I am going to evaluate the immunomodulatory effect of a gram positive probiotic bacteria in vitro and I need to use a positive control. I was thinking about LPS, but it's a component of...

11 March 2020 7,369 3 View

Can we use disposable gavage needles more than one time?

We use currently a steel gavage needle to deliver the substance to our rats. I once saw someone using a plastic needle, and I gave the idea for my advisor, using the plastic needle to lower stress...

02 March 2020 5,971 2 View

What is better to buy a real-time pcr machine or an endpoint?

Hello everyone, we are acquiring a new molecular biology laboratory with the fundamental objective of identifying species of microalgae and cyanobacteria. Perhaps you could help us decide if it is...

24 January 2021 8,320 4 View

If we are going for co digestion of microalgae with wastewater but the sample is having low pH, What are the effective ways to increase the pH ?

wastewater with low pH

07 December 2020 2,716 3 View

Anyone working on Molecular Taxonomy?? I would be highly obliged if anyone could help me with primer selection and Phylogenetic analysis ??

I have a wide range of freshwater microalgae collected from various districts of Tamil Nadu. I am looking for a collaborator who could help me with molecular identification of some rare unknown...

23 September 2020 8,030 1 View

If the microalgae experience stress through transportation. What would be the best course of action to avoid it from becoming contaminated or killed?

I am currently doing an experiment in my faculty lab and I need living micro-algae to be transported from other countries. The samples will be done in Morocco and Spain and they will be sent to...

08 September 2020 4,424 2 View

How can I differentiate between cyanobacteria and microalgae in agar plates?

I'm trying to isolate cyanobacteria from fresh water and soil. I've plated several dilutions of my samples into BG11-agar plates and I have a mixture of green colonies/halos and brown stains which...

06 September 2020 8,959 4 View

How to determine the protein and carbohydrate of microalgae samples?

I used gravimetric analysis to determine microalgal lipid content after lipid extract in my lab. Now we need to determine both protein and carbohydrate content. We have a Velp TKN...

06 August 2020 8,166 4 View

Is it a good idea to build an reactor for Anaerobic Digestion or hydrogen fermentation using acrylic sheets or glass sheet?

Does using a reactor made of acrylic sheets beneficial for microalgae anaerobic digestion?

16 July 2020 9,062 2 View

Microalgae-biofertilizer-application methods?

02 June 2020 5,180 1 View

In Iran which field (macroalgae or microalgae) do you think is more important to spend and why?

Due to the lack of broad algae market in Iran and this image's information, do you think the activities in which the field (macroalgae or microalgae) is more necessary?

13 March 2020 4,887 3 View

What could be the reason for high activation energy during pyrolysis process?

I have a scenario where I used pelleted macroalage for pyrolysis. The heating rates were 20, 30 and 40 C/min. I had experience in using isoconversitional methods in the past were I have seen...

02 March 2020 3,217 5 View