What thickness need for ITO to use as conductive layer?

I want to do RF sputtering of ITO on glass substrate using magnetron sputtering. what thickness do i need to make it good conductive layer to use it for PSC. Moreover, whats about thickness of...

01 March 2021 395 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Special issue on PLS-SEM in Mathematics. Are you interested?

21 February 2021 5,601 3 View

How to calculate the TCID50 when the highest CPE is 50% or less?

I am doing TCID50 to measure progeny virus and some treatments protected the cells in a way that the highest CPE observed is 50% or less. How should I do the titre calculation in that case? Thank you!

16 February 2021 8,768 1 View

Has anyone tried eBioscience Foxp3 / Transcription Factor Staining Buffer Set with TdTomato+ cells?

Hi all, I stained tdTomato+ cells (PBMCs and Jurkats) with eBioscience Foxp3 / Transcription Factor Staining Buffer Set for the foxp3 and I completely lost the signal of tdTomato. I tried to fix...

10 February 2021 5,863 2 View

Which drug is better to modify blood pressure in awake monkey, if I provide it in the food or drink reward?

I need to modify the blood pressure in a monkey while is awake in the recording chamber, and I am planing on provide a drug for that in the food or water used for reward... any advise? Thank you

08 February 2021 9,900 1 View

Why stop the reaction in ice for the fluorescent probe FDA (fluorescein diacetate)?

Hi, In literature I noticed that who stains haemocytes with FDA for 30 min then stop the reaction in ice while this does not apply for 15 min incubation time (or maybe authors did not write this...

08 February 2021 3,366 3 View

How to make Fast Green FCF from powder?

Hi everyone We need Fast Green to electroporate plasmid into mice embryos, therefore we simply need it to see some color in the liquid we are electroporating so that electroporation is more...

07 February 2021 1,671 2 View

Why do high doses of humic substances inhibit the growth of corn roots? Is there a toxicity effect?

Length, volume, surface area and dry mass of corn roots treated with humic substances increased up to 13 liters per hectare and decreased with higher doses.

02 February 2021 2,190 4 View

Using a nucleosil c18 column, i get no negative peaks in real time and two negative peaks in post run.Software problem?

Simadzu, lc solutions

02 February 2021 1,969 2 View

Glass fiber content in a gypsum board ?

Any idea on how to get the volumic ratio of glass fiber in a gypsum board ? Thank you for your help !

01 March 2021 2,501 3 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

How can we limit artifacts caused by rotation in fiber photometry?

We are trying to run fiber photometry experiments for long time scales, but we get these sinusoidal artifacts that come up. We think they may be caused by twisting of the fiber optic subject...

25 February 2021 568 1 View

How to properly calculate Interfacial area density in a porous domain?which are possible value for glass fiber?

I'm doing a cfx simulation of a multiphase flow in pultrusion and i set the Interfacial Area density in the heat exchange of a porous medium in this way IAD=Total fiber lateral surface of fiber...

23 February 2021 6,043 1 View

Suppose we have a banana seller cart consisting of 4 wheels, weighing about 300 kg. How much torque motors are needed to pull of push it electrically?

I am thinking of electrifying the street food carts while harvesting energy using solar panels on top.

22 February 2021 4,323 3 View

How can we add reinforced thermoplastics with granules in injection molding machine?

I need to make an injection molding machine in which short fibers are to be introduced. Short fiber length and material properties are to be determined and also the material strength are to be...

22 February 2021 4,290 1 View

How can I improve the concentration of banana fruits extracted RNA?

Currently, I'm working with banana fruits. I've tried to do RNA extractions with different methods, and I've gotten total RNA without contamination, but I couldn't get a high concentration of...

22 February 2021 6,126 2 View

Staining methods for skeletal muscle fiber typing?

does anyone know the best method to differentiate and quantify rapid fatiguing and fatigue resistant muscle fibers? the old method form the 1980's uses ATP staining which is quite labor intense?

21 February 2021 6,483 3 View

Why the storage moduli E' decrease with highr fiber volume?

Hi guys, I am facing problems to interpret a DMA results. Material: Composite Bisphenol A + curaua fiber What is being tested: the influence of fiber volume on the composites properties...

12 February 2021 174 7 View

What could be the cause to have an E.coli DH10B transformed with pUC18 to grow in MKL + ampicillin but is not LAC+?

Hello everyone, I did a transformation of E. coli DH10B using pUC18 plasmid. The bacterias did grow on MKL medium with ampicillin but the colonies showed a white/pink colour and the medium was...

08 February 2021 9,720 3 View