What statistical analysis method should I use with multivariate categorical data?

Hi, I'm struggling quite a bit, Hope someone can help. My research question is roughly to what extent (and what) characteristics of murder and manslaughter cases are related. The data consists...

05 October 2020 3,958 8 View

Which is the Risk of Long QT and Sudden Cardiac Death by Drugs Used to treat COVID-19?

Numerous drugs are used these days without in-depth evaluation of efficacy and safety for the treatment of life-threatening complications of COVID-19. Among these, an important number are known...

26 March 2020 816 17 View

Which pharmacological modulation of immune response to SARS COV-2 could reduce the incidence of acute respiratory distress syndrome?

Differences in immune responses to emerging corona viruses and the difficulties of developping vaccines contribute to a surge in ARDS in vulnerable populations, leading to acute shortages and...

16 March 2020 3,520 26 View

Does anyone know a freeware tool that can align exon and intron sequences to a template DNA sequence?

I am seaqrching for a tool that can align exon and intron sequences to a template DNA sequence without disrupting any of the short sequences. I remember that DNAStar was able to do this. Does...

12 December 2019 8,902 3 View

Does anyone have any early World History LGBT secondary sources that are non-western?

I am looking to integrate unheard voices into the survey class, before 1500. If you have secondary sources that you are willing to share please let me know.

22 October 2019 1,233 4 View

Taxonomy of Data Sources?

Can anyone suggest a reference that deals with a decent, contemporary taxonomy of data sources? There is the old taxonomy by Cattell: Q-Data (= Questionnaire Data), T-Data (= Test-Data), and...

22 October 2019 8,429 1 View

Are there free online tools for the visualization of gene set (GO-) enrichment analysis results?

Gene set enrichment analyses usually end up with lists of enriched categories associated with a p-value or some othewr measure of reliability. I am looking for free visualization tools such as...

07 January 2019 9,701 1 View

Which staining method to use to quantify low level nerv damage?

02 April 2017 4,332 3 View

Does anyone have a transfection protocol for the NSCLC cell line PC9?

I use HiPerFect from Quiagen. I transfect microRNAs which lead to a reduction of their targets in other cell lines like HCC4011, HCC827 and A549 and siGLO as a control. But I can't detect...

17 February 2017 3,919 3 View

I have a result that generated additional hypotheses, can I include the additional hypotheses and results in the same paper?

Our unpublished results have generated several additional hypotheses. I'd like to keep both the original research goals and the generated hypotheses in the same paper. I was thinking of splitting...

31 December 2016 5,389 24 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View