Rob Ulrich

3 Questions 2 Answers 0 Followers

Questions related from Rob Ulrich

Functional/non specific disulphide bond formation in proteins?

As an alternative to looking at the conservation of cysteine residues amongst homologs of a protein of interest, is anyone aware of a server that can predict whether disulphide bond formation...

22 March 2024 3,791 1 View

Protein precipitates on purification??

I am trying to purify a his tagged protein using IMAC. The homologs of this protein purify very well. However, this protein precipitates on elution. Buffer composition- 20mM Tris-HCl pH7.4, 500mM...

21 March 2024 9,058 4 View

Multiple bands detected in SDS sample of monomer species (gel filtration fraction)??

I have purified several his tagged proteins using IMAC. To clean up the samples further I ran them through a GF column (Superdex 200 increase 10/30Gl). When I ran an SDS sample containing the...

20 March 2024 4,192 3 View