I have a long term stability study with a protein that as an isoletric point lower than the buffer (Tris buffer), and I am seeing a sustantial drift from the initial pH to 12 month point. Temperature, pH meter and other conditions were kept the same.
I have performed some experiments but I am not seeing degradation, or agreegation. Is there other factors involved.
Does the protein have an effect in this pH changes?
John Francis Miller
Have you consider the effect of CO2 dissolving?
Try bubbling an inert gas through a sample and see if the pH changes.
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Andrei Blasko
If you don't see degradation your buffer is wrong. Chose a suitable buffer for your protein, maybe Histidine? Have enough buffering capacity and use degassed solvents as John Francis Miller suggested.
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