Hi Francesco

In our lab we did a colaboration with a facility that performed the protocol as above described:

Adipose tissue (~1 mm3) was fixed with 2% glutaraldehyde for 2 h,

followed by 1% osmium tetroxide for 2 h and 0.5% uranyl acetate over-

night. After dehydration and embedding in resin, ultra-thin sections

(90 nm) were stained with uranyl acetate and lead citrate and analyzed

in JEOL 1010 transmission electron microscope.

You can check the images in the paper:

http://www.sciencedirect.com/science/article/pii/S1388198116300506

Thiago

Thank you very much!!!

Hi, what's the method you used in the last?