How to perform flat embedding with LRWhite for transmission electron microscopy?

Hi all, I'm trying to do flat embedding of monolayer cell culture in LRWhite, but the results are not exciting. I used the "PTFE flat embedding mold" from Ted Pella (attached there is a picture of...

08 September 2018 3,102 0 View

How to perform electron microscopy on cells grown inside a chip?

Hi guys, I will appreciate if somebody could give me some suggestions on how to perform transmission electron microscopy on structures which have been grown inside a chip like...

04 May 2018 9,697 3 View

How to store opened formaldehyde, 16%, methanol free, ultra pure for transmission electron microscopy?

Hi guys, I have some ampules of formaldehyde, 16%, methanol free, ultra pure (Polysciences cat. number 18814-10) and I would like to use them for transmission electron microscopy with...

03 April 2018 4,395 8 View

Porcin skin gelatin for immunogold electron microscopy?

Hi guys, I am doing pre-embedding immunogold electron microscopy. Unfortunately I don't have "cold water fish skin gelatin" as component of blocking agent (along with BSA and NGS). I would like to...

08 September 2017 1,914 0 View

Preembedding immunogold on cell cultures?

Hi guys, I would like to do preembedding immunogold on cell cultures. I followed different protocols and I tried to localize different proteins, with both Nanogold (1.4nm) and Aurion (0.8nm) gold...

06 July 2017 3,780 10 View

What kind of structures in the transmission electron microscopy microglial cells?

03 April 2017 3,768 15 View

How can I fix mouse brain for transmission electron microscopy?

03 April 2017 2,688 7 View

How to properly use the Perfect Loop with slot grids?

Hi guys, I would like to know how to properly use the Pefect Loop in order to pick up ultrathin sections and release them well oriented onto a formvar slot grid. Thank you very much!!! Francesco

31 December 2016 9,671 6 View

Formvar coated grids with wrinkles?

05 June 2016 4,035 2 View

Hayat MA. 2000. Principles and techniques of electron microscopy?

Hi all, I am looking to buy/download the following book: "Hayat MA. 2000. Principles and techniques of electron microscopy", but I did not see any seller in Italy. I will appreciate if someone...

04 May 2016 5,667 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

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02 March 2021 3,060 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View