How to perform flat embedding with LRWhite for transmission electron microscopy?

Hi all, I'm trying to do flat embedding of monolayer cell culture in LRWhite, but the results are not exciting. I used the "PTFE flat embedding mold" from Ted Pella (attached there is a picture of...

08 September 2018 3,102 0 View

How to perform electron microscopy on cells grown inside a chip?

Hi guys, I will appreciate if somebody could give me some suggestions on how to perform transmission electron microscopy on structures which have been grown inside a chip like...

04 May 2018 9,697 3 View

How to store opened formaldehyde, 16%, methanol free, ultra pure for transmission electron microscopy?

Hi guys, I have some ampules of formaldehyde, 16%, methanol free, ultra pure (Polysciences cat. number 18814-10) and I would like to use them for transmission electron microscopy with...

03 April 2018 4,395 8 View

Preembedding immunogold on cell cultures?

Hi guys, I would like to do preembedding immunogold on cell cultures. I followed different protocols and I tried to localize different proteins, with both Nanogold (1.4nm) and Aurion (0.8nm) gold...

06 July 2017 3,780 10 View

What kind of structures in the transmission electron microscopy microglial cells?

03 April 2017 3,768 15 View

How can I fix mouse brain for transmission electron microscopy?

03 April 2017 2,688 7 View

How to properly use the Perfect Loop with slot grids?

Hi guys, I would like to know how to properly use the Pefect Loop in order to pick up ultrathin sections and release them well oriented onto a formvar slot grid. Thank you very much!!! Francesco

31 December 2016 9,671 6 View

Transmission electron microscopy on adipose tissue?

Hi, I will appreciate if someone could give me a detailed protocol for fixation adn embedding adipose tissue for transmission electron microscopy, in order to see mitochondria cristae. Thank you...

08 September 2016 8,336 3 View

Formvar coated grids with wrinkles?

05 June 2016 4,035 2 View

Hayat MA. 2000. Principles and techniques of electron microscopy?

Hi all, I am looking to buy/download the following book: "Hayat MA. 2000. Principles and techniques of electron microscopy", but I did not see any seller in Italy. I will appreciate if someone...

04 May 2016 5,667 3 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View