13 Questions 27 Answers 0 Followers
Questions related from Francesco Boldrin
Hi all, I'm trying to do flat embedding of monolayer cell culture in LRWhite, but the results are not exciting. I used the "PTFE flat embedding mold" from Ted Pella (attached there is a picture of...
09 September 2018 3,160 0 View
Hi guys, I will appreciate if somebody could give me some suggestions on how to perform transmission electron microscopy on structures which have been grown inside a chip like...
05 May 2018 9,773 3 View
Hi guys, I have some ampules of formaldehyde, 16%, methanol free, ultra pure (Polysciences cat. number 18814-10) and I would like to use them for transmission electron microscopy with...
04 April 2018 4,545 8 View
Hi guys, I am doing pre-embedding immunogold electron microscopy. Unfortunately I don't have "cold water fish skin gelatin" as component of blocking agent (along with BSA and NGS). I would like to...
09 September 2017 1,968 0 View
Hi guys, I would like to do preembedding immunogold on cell cultures. I followed different protocols and I tried to localize different proteins, with both Nanogold (1.4nm) and Aurion (0.8nm) gold...
07 July 2017 3,849 10 View
Hi guys, I was observing some microglial primary cultured cells with the transmission electron microscope and I found some structures I never seen. I really appreciate if someone could provide to...
04 April 2017 3,860 15 View
Dear all, A lab asked to our electron microscopy facility to analyze whether antidepressants change autophagy in mouse hippocampus neurons with transmission electron microscopy. In order to do...
04 April 2017 2,788 7 View
Hi guys, I would like to know how to properly use the Pefect Loop in order to pick up ultrathin sections and release them well oriented onto a formvar slot grid. Thank you very much!!! Francesco
01 January 2017 9,726 6 View
Hi, I will appreciate if someone could give me a detailed protocol for fixation adn embedding adipose tissue for transmission electron microscopy, in order to see mitochondria cristae. Thank you...
09 September 2016 8,399 3 View
Hi all, I am using home made formvar coated slot grids, without carbon. I make them in advance from 0.5% formvar in chloroform and then I store them inside a Petri dish. I am encoutering problems...
06 June 2016 4,098 2 View
Hi all, I am looking to buy/download the following book: "Hayat MA. 2000. Principles and techniques of electron microscopy", but I did not see any seller in Italy. I will appreciate if someone...
05 May 2016 5,767 3 View
Hi all, I would like to batch convert simultaneously pictures taken with TIA in a FEI Tecnai electron microscope from ".emi" to ".TIFF" format, instead of doing it one by one. I will appreciate if...
11 November 2015 3,469 5 View
Hi all, I would like to do preembedding immunogold electron microscopy on muscle. Do anyone have a protocol? Thank you very much!!!
07 July 2015 7,466 0 View
