Has anyone experiences with the Qiagen Epitect DNA bisulfite Kit and with precipitates in BL buffer? I incubated a flask of BL at 60°C for 3 hours, and precipitates are still there. I found information about temperature in troubleshooting, but there is no additional information about the ideal time of incubation.
I used the same kit a few years ago and while using it never noticed something like that. In my case the kit worked fine while using DNA obtained from blood and tissue samples.
If possible i'll recommend you to contact the provider as soon as possible. Meanwhile, you could carry out some tests, but for that, methylation controls (completely methylated and unmethylated) are crucial, just as well-designed primers for unbiased amplification of DNA regions independently of their methylation state.
If you need further advice regarding this kit and or methylation assays, let me know. I have some experience with bisulfite sequencing and methylation sensitive restriction digestion.
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