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Questions related from Mauricio de la Rosa-Garza
What could i use to inactivate COVID19 swab samples contained in viral transport media without compromising compatibility with Viral RNA extraction kits (e.g. Qiagen Viral RNA mini kit)? I'm well...
01 June 2021 8,057 3 View
Hi everyone. I'm currently working with several datasets with no replicates to find genes of interest. I have seen in other questions from RG that this issue has been mentioned several times....
11 August 2020 4,372 0 View
I have few experience working with AN3CA cells. I seeded single cells in a 96 well plate and many of the wells have these structures and they are found with or without colonies of AN3CA that came...
10 May 2020 9,060 1 View
I received a vial of cryopreserved LnCaP cells from a colleague and they didn´t attach to the T25 flask. After that i asked for another vial and this time i coated the flask with poly L Lys and...
12 October 2018 2,693 2 View
I´ve been having a lot of contamination problems when carrying out assays at "high" enzalutamide concentrations (>100uM) in cell culture media. I´ve carried out different tests to determine which...
01 July 2018 7,795 3 View
I´m currently trying to determine the ic50 of enzalutamide in several cancer cell lines. But i have many doubts and currently i´m the only one working with this kind of assays in my...
29 April 2018 9,710 6 View
I ordered some breast cancer cell lines from ATCC (CAMA-1, SKBR3, MDAMB453), but i don´t have the media that atcc recomend to culture those cell lines (EMEM, McCoy's 5a Medium Modified and L15,...
04 April 2018 7,502 4 View
The normal color of the media after being prepared with water, sodium bicarbonate and FBS should be an intense red color (as far as i know), but this time the color of the media is still red but...
17 October 2017 7,494 0 View
I was wondering if quiescent cells will have enough metabolic activity to interfere with the interpretation of the results (% of growth inhibition), in other words, if the formazan production...
18 August 2017 8,466 0 View
I was wondering if this is possible and which could be some drawbacks of using this approach, considering that the drug is an antiandrogen and it works mainly as a growth inhibitor.
02 August 2017 899 1 View
Which housekeeping gene could I use for relative expression analysis in mature rat sperm?
04 May 2017 3,246 3 View
Hi everyone Could someone give me an example of micronutrient-derived loss of imprinting in which the actual change is the hypermethylation of the non-imprinted allele or the hypomethylation of...
27 April 2017 370 2 View
Hi everyone I want to know if the methylation status of both IGF2 alleles will be the same across the different tissues (imprinting) or will it change depending on its source in a "normal" adult...
24 April 2017 3,263 2 View
I´ve been making a little research about resistance mechanisms in cancer and i got to this question. I thought that maybe this resistance is established by the LD50 of the drug since it would be...
28 February 2017 1,903 1 View
Reading many papers I´ve found that in this context (cell proliferation) using metabolic assays such as MTT is like "the gold standard". Many papers use this approach when trying to determine...
01 January 1970 6,090 4 View
I´m struggling with deciding which media to use (I´m new in this cell culture field). Rigth now i have MEM and DMEM media on the lab, but i´ve seen that in some articles they use specific media...
01 January 1970 8,638 4 View
In a proliferation experiment, i´ve read in many places that a positive control could be anything that "kills" all the cells (best case scenario will be the lowest concentration of your tx at...
01 January 1970 2,832 4 View
