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Questions related from Mauricio de la Rosa-Garza
What could i use to inactivate COVID19 swab samples contained in viral transport media without compromising compatibility with Viral RNA extraction kits (e.g. Qiagen Viral RNA mini kit)? I'm well...
01 June 2021 7,992 3 View
I have few experience working with AN3CA cells. I seeded single cells in a 96 well plate and many of the wells have these structures and they are found with or without colonies of AN3CA that came...
10 May 2020 8,875 1 View
I ordered some breast cancer cell lines from ATCC (CAMA-1, SKBR3, MDAMB453), but i don´t have the media that atcc recomend to culture those cell lines (EMEM, McCoy's 5a Medium Modified and L15,...
04 April 2018 7,314 4 View
The normal color of the media after being prepared with water, sodium bicarbonate and FBS should be an intense red color (as far as i know), but this time the color of the media is still red but...
17 October 2017 7,432 0 View
Which housekeeping gene could I use for relative expression analysis in mature rat sperm?
04 May 2017 3,039 3 View
Reading many papers I´ve found that in this context (cell proliferation) using metabolic assays such as MTT is like "the gold standard". Many papers use this approach when trying to determine...
01 January 1970 6,028 4 View
I´m struggling with deciding which media to use (I´m new in this cell culture field). Rigth now i have MEM and DMEM media on the lab, but i´ve seen that in some articles they use specific media...
01 January 1970 8,584 4 View
In a proliferation experiment, i´ve read in many places that a positive control could be anything that "kills" all the cells (best case scenario will be the lowest concentration of your tx at...
01 January 1970 2,781 4 View
