Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Species identification.Who can tell me the scientific names of these Gastropods?

Species identification. I've been doing research on communities of marine macrobenthos recently, but some of them can't be identified, which hinders my research. Who can help me identify these...

14 December 2020 8,357 3 View

Ideas for Discussion of a Case-study analysis?

I am currently researching the demotivating factors and remotivating factors of high school students in my country. My study only has 13 participants, and I groups them into 3 groups depending on...

02 December 2020 2,288 3 View

About cross-case analysis?

Currently, I have finished interviewing my high school students to learn about their demotivating and remotivating factors. I'm trying to use case-study method to analysis data. In my...

17 November 2020 7,989 2 View

I alway get serval big plaques even with very high concentration of bacteriophages. how to get well-distributed plaques with double layer agar method?

I used MS2 (ATCC 15597-B1) strain to do my experiment. I always get serval big plaques (like the picture I attached), but not a plate which has a lot of small plaques I saw from other research....

15 November 2020 7,488 4 View

Dear All, could you please show me how should I do to open SIR-C compressed image with ENVI 5.3 or ArcGIS 10.4 softwares?

I would like to apply a remote sensing processing in this file for a structural geologic feature study. The compressed file name: PR48120.tar The three unzipped files which come from PR48120.tar...

23 September 2020 4,924 2 View

How can I make the Gold-Antibody conjugate stable ?

I did competitive lateral flow immunoassay using gold bridge method. Antigen-fluorescence bead and immobilized antigen on the PMMA membrane is no problem to measure the optical absorption at 37...

24 August 2020 2,684 4 View

About researching EFL learning demotivation and Straussian grounded theory?

Currently, I am conducting a MA dissertation by using Strauss and Corbin's Grounded Theory. My topic is researching EFL learning demotivation of high school students (16 to 18 years old). My main...

13 July 2020 6,773 4 View

How to represent damping in a system with nonlinear stiffness?

We all know that damping can represent by Damping ratio and Natural frequency . We can represent the damping in terms of ( ''Linear stiffness - The first term in the nonlinear stiffness )...

25 May 2020 6,323 7 View

What kind of optimization is done through genetic algorithm in architecture?

Difficulties and types of optimization

11 May 2020 6,177 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

How to choose ANN number of hidden layers and nodes?

Hello, Could you please share any interesting research explaining how to choose the number of hidden layers and nodes per layer in case of regression problems using ANN? Thank you, any help would...

01 March 2021 6,200 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

How to improve and implement WBAN MAC and Radio modules under Castalia 3.2 ?

Hello researchers, Hello Castalia3.2 researchers I am a beginner with Castalia, I work on optimization of RFID systems used in wireless body networks. I simulated a WBAN with 10 nodes and one...

28 February 2021 400 1 View

How could I take into consideration the weights of observation in a system of seemingly unrelated regressions (SUR) in rstudio?

I build a model of 4 regression equations (probit and OLS) in R studio. I need to take the weights of observations into consideration. While it is simple to do so for every regression equation,...

27 February 2021 1,233 1 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Can random sampling have inclusion criteria?

As random sampling means that everyone in the population have an equal chance to be chosen, can it have inclusion criteria? My research participants have been chosen based on the inclusion...

26 February 2021 6,905 10 View