I used NHS-PEG4-Azide to label protein and temporarily store the labelled protein at 4 degrees. Is it suitable for long-term storage at this temperature?
For long-term storage of NHS-PEG4-Azide labeled proteins, it's better to freeze them at -20°C or ideally at -80°C, rather than storing at 4°C. Lower temperatures slow down degradation processes, preserving the protein's structure and function for extended periods.
In general, one of the commonly used storage conditions for azide-labeled proteins or peptides is to keep them in buffer solution at a low temperature, usually at -20°C or even lower, such as -80°C. This helps slow down any potential degradation reactions and maintain the integrity of the labeled molecules. In addition, storing them in the dark can prevent any photochemical reactions that may occur... On the other hand, storing azide-labeled proteins or peptides at low temperatures in a suitable buffer solution and protecting them from light should help maintain their preservation. Its stability over time.
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