My vector in which I cloned shRNA primers have a very high copy. I cloned shRNA plasmids and then tried to transform them in Stbl4 but I'm getting very very low or almost negligible concentration of DNA by both Mini prep & MEdi prep. I need DNA to proceed with lentiviral production. What could be the reason for this problem? Bacteria are growing fine in LB broth and it turns turbid too.

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