Mikel Irastortza-Olaziregi

3 Questions 6 Answers 0 Followers

Questions related from Mikel Irastortza-Olaziregi

Is it possible to elute formaldehyde-crosslinked FLAG-tagged protein-RNA complexes from FLAG resins using high salt buffers?

I routinely purify FLAG-tagged protein-RNA complexes that have been crosslinked with formaldehyde using FLAG peptide. Alternatively, it is possible to elute proteins from FLAG resins using high...

04 March 2019 6,695 3 View

Screenings for genes involved in biofilm formation: any suggestions?

In our lab we investigate the biofilm formation of uropathogenic E. coli and I would like to perform a random mutagenesis experiment to be able to identify genes mediating this process. However,...

16 November 2017 7,818 2 View

How to reduce protein aggregation during protein concentration?

I am trying to concentrate the secretome of E. coli using Vivaspin columns (GE). I succesfully concentrate the proteins but when running SDS-PAGE I observe pretty blurry bands and irregular...

21 June 2016 530 9 View