Disha Chauhan

4 Questions 32 Answers 0 Followers

Questions related from Disha Chauhan

Primary Rat Neuron Culture of E17-E18 are dying after 24-48 hours

Hi, we have been doing cortical neuron culture from E-17-18 Embryos of rat and so far all has been good but all of sudden we are having a strange phenomena that after 3 days or sometimes 2 days of...

23 March 2017 4,960 4 View

Plasmid DNA isolation for shRNA vectors giving very low yield with Stbl4 transformation

My vector in which I cloned shRNA primers have a very high copy. I cloned shRNA plasmids and then tried to transform them in Stbl4 but I'm getting very very low or almost negligible concentration...

08 April 2013 3,935 15 View

Whats the best way to transfect Hela Cells? We are using PEI but efficiency is very low.

We are using Hela cells for checking localization of proteins by Immunofluorescence and transfecting these cells with PEI, but efficiency is just 30 %. How to improve it? Should we use another...

25 September 2012 2,729 16 View

Double In situ Hybridization

I need to do Double In situ Hybridization , is anyone having optimized protocol for it , Please help me with this , we have been doing In situ hybridization in our lab using DIG labelled probe...

21 April 2012 2,195 2 View