Is there some sort of classification of simple algebras with color involution?

If $A$ is a G-graded algebra then one can define on it a color involution, i.e. a bijective linear map preserving the grading such that the image of a product of two homogeneous elements is...

24 September 2020 5,416 3 View

Electrochemistry in Organic chemistry, Book article review?

Hello, I'm starting a new project that involve the use of Electrochemistry in organic synthesis. For this reason i'm searching for a book to have an introduction on the topic as also some review...

30 November 2019 3,789 4 View

Stable plant miRNAa as qPCR normalizers for Taqman Advance protocol?

We are going to use custom TaqMan TM Advanced miRNA Assays for microRNA quantification experiments in plants. These assays are not compatible with the use of U6 snRNA as a reference internal...

02 November 2017 4,545 2 View

How do I measure / evaluate / report about the learning effect of repeated time measurements?

I conducted an experiment with 80 subjects, each of them performing 50 trials. I measured the time (in seconds) needed to accomplish each trial. Trial-after-trial, every subject has the tendency...

11 December 2016 9,145 6 View

Issue with protein overexpression?

Hi, I am using a lentiviral vector to over express a protein in AML cells. I have cloned the flag-gene into the EIFalfa-MCS-SV40 vector. When I use this vector to simply transfect 293 cells, I can...

06 October 2016 903 3 View

ChIP-qPCR: same volume or same DNA concentration?

21 September 2016 4,154 4 View

How many votes are needed in a study based on Paired Comparison?

I am running a study involving N=100 items that subjects will compare, side-by-side, and declare the preferred one. This process is known as Paired (or Pairwise)...

14 July 2016 8,120 3 View

Chip-seq. IgG and IPs have the same DNA concentration?

Hello, I'm doing a Chip-seq experiment and I've noticed that after DNA extraction I have the same amount of DNA in my negative control (IgG) and in the IPs? Do you think it's still worth it to...

27 February 2016 8,127 6 View

How to assess the sexual maturation in wild long-lived birds when ageing is not possible??

Cloacal or blood sampling? What kind of biomarker (testosterone, sperm, other hormones...) ?

24 March 2015 893 6 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

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03 March 2021 5,360 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View