I normally use this protocol for ChIPseq:
https://www.nature.com/articles/nprot.2006.98
After chromatin sonication, it says to add 1/10 volume of Triton X 10% to the samples, spin to remove pellet debris and then incubate beads/chromatin o/n.
What does the triton do in this step?
Thanks
Whenever triton X-100 is used, it is mainly for the lysis of cells. It is commonly used in a wide range of techniques that involve protein isolation
Shawn Krueger cells are already lysed at that point, cause it is after sonication
I think it's just to ensure proper degradation of your cells. Your cells should be lysed by the sonication, but this may improve or ensure cell membrane break down.
Is your next step the addition of proteinase K? Triton-X in solution with proteinase K improves the efficiency of this enzyme.
Fabrizio Simeoni
To bring back this question in a little more detail. Triton X-100 helps lyse cells, makes membranes permeable, and stabilizes proteins. Triton X-100 is a very hydrophobic molecular that also helps solubilize proteins into solution. The permeability allows hydrophillic reduction-oxidation molecules to pass through the cell membranes. More detailed information can be found below:
https://www.pnas.org/content/107/39/16783
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