I tried to reactivate an ME49 cryotube in an LLCMK2 monolayer (epitelial kidney cells). The first days we had growth so we were able to infect mice with this strain, and it was succesfull (we could get tissue cysts). But after a couple of weeks we were not able to see any tachyhzoite or cyst in the monolayer. I kept the culture for a month but i couldn't see anything in the moonolayer, indeed the mk2 continued to grow and there were no signs of lysis caused by the tachyzoites . The cell line may be causing this? Do i have to change to a fibroblast cell-line-type? The culture conditions are: RPMI 2% SFB, 2 mM L-glut, 1 mM non-essential aminoacids, 1mM sodium piruvate, CO2 5% 37°C