Fabrizio Cillo

4 Questions 5 Answers 0 Followers

Questions related from Fabrizio Cillo

Plant RNA extraction kit - Washing solution?

Dear all, we are using Norgen's Plant/Fungi Total RNA Purification Kit for our extractions. As often happens, we run out of washing buffer but there are still a lot of columns that might be used....

23 February 2023 1,362 2 View

Stable plant miRNAa as qPCR normalizers for Taqman Advance protocol?

We are going to use custom TaqMan TM Advanced miRNA Assays for microRNA quantification experiments in plants. These assays are not compatible with the use of U6 snRNA as a reference internal...

03 November 2017 4,700 2 View

Best protocol for RNA extraction from oil matrices?

I am looking for an efficient protocol for extracting total RNA from olive oil or other plant-derived oils for human nutrition. Thank you for the support, kind regards.

22 December 2016 5,054 3 View

Has anybody used, or is aware of the existence of, an anti-PDS antibody that would work well in western blot on plant proteins?

Phytoene desaturase (PDS) is commonly used as a reporter gene for RNA silencing experiments in plants. I would be very pleased to receiving from people that produced/bought an anti-PDS antibody a...

24 October 2014 8,947 1 View