Hi everyone Idisolved my drug 3mg in 1ml DMSO and I take 20uL from it and I dissolved in 10ml cell culture media then I make serial dilution 2 fold and I did the same thing with DMSO. But it show me the DMSO kill the cells rather than my drug
Hello Sb Amiyah.
Have a look at the similar topic Q&A. You will get a pretty confident idea.
https://www.researchgate.net/post/What_is_the_best_protocol_to_detect_ROS_in_cell_culture_using_CM-H2DCFDA
https://www.researchgate.net/post/What_is_proper_concentration_of_calcium_ionophore_A23187_for_induction_of_acrosome_reaction_of_human_sperm
https://www.researchgate.net/post/if_CSCs_are_not_responded_to_the_extract_administered_to_them_till_6_mg_what_is_the_next_step
Regards.
10 micro molar DMSO is ideal.
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