I have used Hygromycin B in Hela cells. Doesn't seem to be selecting. I am using pcDNA 4TO vector and hoping to do the selection.
I need a thesis on the above topic
28 July 2024 3,867 1 View
I have work on comparative analysis of different shampoo, which prove its better anti- Dandruff activity, i refer in many papers only fungal isolates (Malassezia Yeasts) are used to showing anti -...
16 February 2024 3,911 3 View
I Synthesized Nvoc-PEG-NH2 , each time I try to isolate the compound from diethyl ether via vacuum filtration it absorbs moisture and starts to turn to a liquid and I end up loosing most of my...
23 October 2023 7,623 0 View
I want to assess burnout syndrome among healthcare professionals during COVID-19 pandemic and I need too----MBI-HSS.
22 July 2020 2,992 9 View
I’m working with HEK293T cells and all of the sudden the cells started to die rapidly. There is a lot of floating cell debris but also these weird little fibre structures. I thought it might be...
01 July 2020 5,946 2 View
Currently working on the theory of American humor and satire, making a list of some national specifics of them
20 April 2020 5,418 1 View
All women have the right to a safe and positive childbirth experience, whether or not they have a confirmed COVID-19 infection. But, what is happening in reality? Please add an answer and visit...
30 March 2020 3,098 4 View
Hi everyone, I have some issues to determine how to pick correctly the right distance calculation method in R. I have two datasets : one containing habitat variables (worldclim data, so...
15 October 2019 7,690 5 View
Hi guys, I'm using DIPEA/HOBt to couple my amides. The products are there but there are unnecessary intermediates or spots that appearing on the TLC plate. I tried filtering & flash...
14 August 2019 1,542 4 View
If you have the design guidelines for clutch housing with ventilation concept, kindly share me the guidelines. For my project heat dissipation is more. So we are planning to ventilation in the...
18 February 2018 3,609 0 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
Dear Researchers I need to know, how to load and plot 2D-PIV (particle image velocimetry) recorded velocity vector field data in Tecplot? Thank You
02 August 2024 3,615 1 View
I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...
01 August 2024 7,677 2 View
What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?
31 July 2024 4,835 3 View
Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...
31 July 2024 3,893 0 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View