What is the ethical procedure to collect samples from human such as blood, tissue, sperm for genome sequencing?

Hello All Can you share your knowledge, the ethical issues for collecting the sample from the human ( Blood, tissue, sperm)

04 May 2018 6,372 0 View

Is any sequences of Viral lncRNA reported?

Hello I want reported sequences of viral non coding RNA of any virus. Is there any database or literature available so far.

03 April 2018 1,644 1 View

Coding (lnc RNA) potential of viruses?

How viruses transferring their genetic information gene to protein and how it produce lnc RNA to suppress or activate a specific genes in the host system. What are all the major difference of...

02 March 2018 2,314 0 View

How to compare time series data of gene expression studies?

Hello All I have a time series micro-array data, the format look likes, patient samples collected from Day 1 to Day 40 ( sample size 40)and control sample ( 20). I would like compare all the...

11 December 2017 2,559 3 View

How NGS applied in the field of virology? What are all the merits and demerits?

08 September 2017 1,031 0 View

How to analyze large samples using cytoscape(MCODE)?

05 June 2017 8,371 2 View

Comparison of microarray data of Human and NHP is reliable?

11 December 2016 141 0 View

Data mining applications in viral microarray datasets?

Hello every one, I looking for the application of Data mining concepts in Viral micro-array data sets.can you suggest any paper dealing with above mentioned.( not looking for paper related virus...

04 May 2016 1,370 0 View

Meta analysis of microarray data merits and demerits?

Hello , Meta analysis of micro-array data may help to identify novel insights of gene expression data sets.Most of the meta analysis work form cancer studies. can i use this methodology for viral...

02 March 2016 4,192 1 View

Down regulated genes?

Can you share the paper only discus about the down regulated genes? How to set the parameter to get the down regulated genes What is p value and corrected p value

02 March 2016 5,798 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View