Hello
I want reported sequences of viral non coding RNA of any virus. Is there any database or literature available so far.
Please, see below: http://www.rna-society.org/virbase/
Hello All Can you share your knowledge, the ethical issues for collecting the sample from the human ( Blood, tissue, sperm)
04 May 2018 6,474 0 View
How viruses transferring their genetic information gene to protein and how it produce lnc RNA to suppress or activate a specific genes in the host system. What are all the major difference of...
02 March 2018 2,444 0 View
Hello All I have a time series micro-array data, the format look likes, patient samples collected from Day 1 to Day 40 ( sample size 40)and control sample ( 20). I would like compare all the...
11 December 2017 2,667 3 View
Hello experts This is very common question Next generation sequencing now applied almost all biological field in including virology, the data publically available like WGS of virus and viral...
08 September 2017 1,161 0 View
Hello I am doing gene network analysis using micro array data set for identifying the gene enrichment,pathway and transcription factor analysis. My data set contain 79 sample with different time...
05 June 2017 8,491 2 View
hi to all can you give me your suggestion , Is the Human and Non human primates microarray data is reliable to compare in the context to understand the difference in gene network modules
11 December 2016 257 0 View
Hello every one, I looking for the application of Data mining concepts in Viral micro-array data sets.can you suggest any paper dealing with above mentioned.( not looking for paper related virus...
04 May 2016 1,486 0 View
Hello I want to do pathway analysis of list of miRNA which are related to multi drug resistance, can you Share any ideas about the suitable tools?
03 April 2016 7,593 1 View
Hello , Meta analysis of micro-array data may help to identify novel insights of gene expression data sets.Most of the meta analysis work form cancer studies. can i use this methodology for viral...
02 March 2016 4,339 1 View
Can you share the paper only discus about the down regulated genes? How to set the parameter to get the down regulated genes What is p value and corrected p value
02 March 2016 5,899 3 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
The stability of the Solar System is a complex subject that blends the classical framework of Newtonian mechanics with the modern insights provided by General Relativity (GR). Understanding this...
07 August 2024 2,569 1 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View