Is any sequences of Viral lncRNA reported?

Hello I want reported sequences of viral non coding RNA of any virus. Is there any database or literature available so far.

03 April 2018 1,644 1 View

Coding (lnc RNA) potential of viruses?

How viruses transferring their genetic information gene to protein and how it produce lnc RNA to suppress or activate a specific genes in the host system. What are all the major difference of...

02 March 2018 2,314 0 View

How to compare time series data of gene expression studies?

Hello All I have a time series micro-array data, the format look likes, patient samples collected from Day 1 to Day 40 ( sample size 40)and control sample ( 20). I would like compare all the...

11 December 2017 2,559 3 View

How NGS applied in the field of virology? What are all the merits and demerits?

08 September 2017 1,031 0 View

How to analyze large samples using cytoscape(MCODE)?

05 June 2017 8,371 2 View

Comparison of microarray data of Human and NHP is reliable?

11 December 2016 141 0 View

Data mining applications in viral microarray datasets?

Hello every one, I looking for the application of Data mining concepts in Viral micro-array data sets.can you suggest any paper dealing with above mentioned.( not looking for paper related virus...

04 May 2016 1,370 0 View

Pathway analysis of miRNA?

03 April 2016 7,482 1 View

Meta analysis of microarray data merits and demerits?

Hello , Meta analysis of micro-array data may help to identify novel insights of gene expression data sets.Most of the meta analysis work form cancer studies. can i use this methodology for viral...

02 March 2016 4,192 1 View

Down regulated genes?

Can you share the paper only discus about the down regulated genes? How to set the parameter to get the down regulated genes What is p value and corrected p value

02 March 2016 5,798 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View