Dear all,
I am wonder if it is differ for activation of ALZET micro osmotic pump at drying oven ( like glassware sterilization ovens, 37 celcius degree) or typical cell culture incubator with humidity. Where do you incubate those pumps?
Thank you.
Srinivas Jagarlapoodi
Fill the pumps in the usual manner. Place the prefilled pumps in sterile 0.9% saline or PBS at 37º C for at least four to six hours (preferably overnight) prior to implantation. (Exception: Model 2004 requires 40 hours to prime, Model 2006 requires 60 hours to prime, Model 2001D requires 3 hours, and Model 1004 requires 48 hours.) If using a catheter, it is possible to drape the end of the catheter outside the beaker to avoid any mixing of solutions. Do not be concerned if due to evaporation, fluid is not observed dripping from the end of the catheter, as evaporative loss can occur. Remove the pump from the saline and implant immediately. http://www.alzet.com/products/guide_to_use/filling.html
you can do priming in a cell culture incubator (as it is more sterile to that of a oven) preferably without humidity as there is every chance that excess humidity may activate the pump.
Priming Procedure:
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Vijayakumar Sukumaran
Fill the pumps in the usual manner then place the pre-filled pumps in sterile 0.9% saline or PBS at 37 ºC for at least four to six hours prior to implantation.
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