Muge Atis

5 Questions 11 Answers 0 Followers

Questions related from Muge Atis

Osmotic pump incubation before implementation at drying oven or cell culture incubator both sterile conditions?

Dear all, I am wonder if it is differ for activation of ALZET micro osmotic pump at drying oven ( like glassware sterilization ovens, 37 celcius degree) or typical cell culture incubator with...

09 September 2018 9,701 2 View

Does expired human collagen(LYOPHILIZED) type IV work while coating plate?

The tube has net been opened yet.

04 April 2017 4,429 1 View

Is there any suggestion on which buffer to use to keep cell culture alive for few hours while working at room temperature w/o CO2 supply?

I know two kinds of buffer.First one is recording buffer containing mM: 110 NaCl,5.4 KCl,1.8 CaCl2, 0.8 MgCl2, 10 D-glucose and 10 HEPES pH:7.4. The second is 1xTris buffer saline with mM 1.5...

06 June 2015 5,774 8 View

Wrong template but have a reasonable PCR product?

Can it be possible to have a PCR product from a wrong backbone? Primers of that PCR protocol were designed with respect to backbone sequences downloaded from a database. However, we realised that...

09 September 2014 1,768 10 View

No colony after ligation - can anyone help?

Mostly, the DNA concentration of the pcr product after gel isolation is 10-17ng/ul (there is no peak at 260nm, even I used Qiagen kit). I am sure that I cut the right band from the agarose gel and...

08 August 2014 8,959 7 View