Why does my MTT Assay remain in purple color and does not change to yellow?

19 January 2021 5,098 4 View

What indicators are suitable for measuring someone's experience?

Hello everyone, Sometimes we feel confused when we want to focus on measuring or assessing someone's experience, because someone's experience has a very broad scope, both morally and spiritually....

05 January 2021 584 3 View

What kind of detector is used in the double slit experiment to determine through which slit the photon or electron passes?

Explain exactly what the detector does and if the choice of detector affects the outcome. Einstein and Bohr had some discussions on this. What's the current situation?

29 December 2020 1,984 5 View

staircase ramp using VPWL in cadence ?

how to generate staircase ramp using VPWL signal in cadence ?

27 December 2020 1,521 3 View

Can photons curve the spacetime and how would you measure this effect?

Mass bends spacetime. It's generally accepted that the photons have no mass, but they do have momentum and energy and can cause pressure, so they have equivalent mass of p/c. The...

14 December 2020 3,195 6 View

Ways to establish exchange bias in ferromagnet/ antiferromagnet heterostructure?

When ferromagnet (FM) and antiferromagnet (AFM) are coupled, exchange bias can be generated at the interface of FM and AFM which acts as a bias field in the hysteresis loop. I am particularly...

24 October 2020 8,580 1 View

How does a single H3 mutation capable of affecting the global level of H3K27me3?

In DIPG patients, one puzzle is that typically only one copy of the H3 gene is mutated. Therefore, how does one single copy of mutation affect the global level of H3K27me3, considering that there...

04 October 2020 3,578 1 View

How to calculate Lexical frequencies and Text Frequencies of phonemes and graphemes of English language?

This is so far the procedure I was trying upon and then I couldn't fix it As per my understanding here some definitions: - lexical frequencies, that is, the frequencies with which correspondences...

27 September 2020 4,742 3 View

How heat generated during friction stir welding due to friction and plastic deformation can be modeled by Abaqus?

I want to make FSW simulation By Coupled Eulerian Lagrangian (CEL) method by Abaqus software. My question is how I cane consider the heat generated in the modle step by step on Abaqus.

10 June 2020 8,428 1 View

What is the Algebra structure ?

I have proved some property. 1. Idempotency, 2. Commutativity, 3. Associativity, 4. Absorption law, 5. Distributivity and 6. De Morgan's laws over complement. what is the structure of this...

28 April 2020 9,188 4 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to get started building and analyzing a UTAUT model?

Hello all, I need help getting started building and analyzing a UTATU model. I have a survey ready with some questions but I had some confusions on UATAUT and on how to analyze the data. I will...

02 March 2021 9,271 1 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Could anyone tell me what is the range of submillimeter wave?

There is confusion regarding the submillimeter-wave range. Many papers specify its range from 300GHz but some also consider its range from 100GHz. If I design an antenna on 122ghz what would I...

01 March 2021 516 7 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View

How the values of errors can be calculated for thermodynamic data?

I have calculated thermodynamic parameters for surfactant solutions in aqueous and mixed alcoholic solvents. I want to calculate the value of errors in the data and represent it in the table along...

22 February 2021 239 2 View