I have a fluorophore and its emission is quenched on adding analyte compound. The overlapping spectra of compound and analyte make UV-vis absorption analysis unreliable.
The analyte mixture spectra shows larger absorbance value than fluorophore compound.
Although I depend on emission spectroscopy for the quenching constants and etc.
I'm curious to know if there are any possible computing methods to overcome this problem and making the absorption spectrums useful for my purpose (explaining emission quenching)?
Four novel spectrophotometric methods, induced dual wavelength method (IDW), dual wavelength resolution technique (DWRT), advanced amplitude modulation method (AAM), and induced amplitude modulation method (IAM), are reliable for determining overlapping spectral components in binary mixtures.
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