Nuclear extraction of mouse liver?

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Derivation of S-parameters measurement equation?

Hi community, I am doing my phd in Nanoelectronics, I just started it and I am doing Bibliography part. With regard to this I was learning about the 12 term error term model for 2 port network...

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Where can I get image datasets of dark skin diseases?

I am doing research on diagnosis for dark skin people. However it's really difficult to acquire the dark skin disorders’ images, please tell me the ideas to get the dark skin disorders’ images...

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Is it feasible to use CVD method to epitaxy ohmic contact electrode for n-type GaN?

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How to extract cAMP from mouse liver?

Dear all, I would like to ask the method to extract cAMP from mouse liver. I found some papers homogenizing the tissue in HCl. Is this the most appropriate method? Thanks

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AAV purification from HEK293A cells?

Hi all, I am producing AAV vector-containing shRNA to knockdown my target protein. Three pDNAs (transfer plasmid, pHelper, RepCap) are transfected into HEK293A (80% confluency). I can get a very...

27 November 2019 2,339 6 View

The target location of siRNA?

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Mouse primary hepatocyte isolation?

Hi all, I am writing this question to inquire about the isolation of mouse primary hepatocytes. As I am a completely beginner, after isolating the cells, it turn out that the viability is 50%...

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Using cAMP+Dexamethasone to mimic the signaling of Glucagon

Hello all, I hope all of you are doing fine. As we know well that Glucagon and Glucocorticoid activate hepatic gluconeogenesis via cAMP. I found a paper using cAMP/dex to treat to cultured cell...

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Low amplification efficiency in qRT-PCR

Hello everyone, I am conducting the qPCR to check the gene expression. In spite of high reaction efficiency of the housekeeping gene, my gene of interest amplifies very low as the ct value is...

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Amplicon length for qpcr using SYBR Green

I am conducting the qpcr to check the gene expression using SYBR Green. After reading through many articles, I came to the conclusion that the optimal amplicon length for qpcr using SYBR Green is...

29 May 2017 871 15 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

The question is how to use Wavenet transform?

HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?

03 August 2024 7,829 0 View

How to correctly use the UTE and ZTE pulse sequences in Bruker's ParaVision software?

I am using a Bruker 600M solid-state NMR spectrometer with a Micro 2.5 microimaging system. The test sample is a tube of 1M LiCl aqueous solution, and the nucleus detected is 1H. I am trying to...

01 August 2024 9,227 1 View

Tissue homogenization in mouse with FastPrep-24™ 5G - is the D matrix system useful?

I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...

31 July 2024 9,186 0 View

Can i use the protease inhibitors during cell membrane vesicle preparation ？?

I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...

30 July 2024 7,077 1 View

IHC profiler in image j software. has anyone used it to quantify nuclear DAB positivity?

Dear researchers. I tried using the IHC PROFILER in image j to quantify nuclear DAB staining. I followed the instructions in the original article by "Varghese F, Bukhari AB, Malhotra R, De A...

29 July 2024 2,229 0 View

How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ?

Greeting How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ? Are 100 events count sufficient and three repetitions for...

25 July 2024 7,520 0 View