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Questions related from Jojo Redb
Dear all, I would like to ask the method to extract cAMP from mouse liver. I found some papers homogenizing the tissue in HCl. Is this the most appropriate method? Thanks
13 March 2020 581 1 View
Hi all, I am producing AAV vector-containing shRNA to knockdown my target protein. Three pDNAs (transfer plasmid, pHelper, RepCap) are transfected into HEK293A (80% confluency). I can get a very...
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Hello all, I want to knock down the gene using siRNA. As siRNA of my targeted gene is commercially available in life technologies. Although there are many candidates available, there possess...
30 January 2018 7,611 4 View
Hi all, I am writing this question to inquire about the isolation of mouse primary hepatocytes. As I am a completely beginner, after isolating the cells, it turn out that the viability is 50%...
09 November 2017 6,626 7 View
Hello, I would like to inquire if anybody has the protocol for nuclear extraction of mouse liver. Thanks
25 October 2017 8,765 3 View
Hello all, I hope all of you are doing fine. As we know well that Glucagon and Glucocorticoid activate hepatic gluconeogenesis via cAMP. I found a paper using cAMP/dex to treat to cultured cell...
14 August 2017 9,188 0 View
Hello everyone, I am conducting the qPCR to check the gene expression. In spite of high reaction efficiency of the housekeeping gene, my gene of interest amplifies very low as the ct value is...
30 June 2017 9,782 3 View
I am conducting the qpcr to check the gene expression using SYBR Green. After reading through many articles, I came to the conclusion that the optimal amplicon length for qpcr using SYBR Green is...
30 May 2017 812 15 View
