Normal cell line for comparison for liver cancer cell line

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Did anybody worked with EphA family protein expression? MW 130kDa . I am not getting any expression. Do you use different lysis buffer or RIPA?

I am working on EphA2, pEpA3 expression with different cell types (MD-MBA231, MOLT4) for the expression of EphA family proteins, They are of high molecular weight 130 kDa. I use RIPA buffer and...

01 December 2020 8,610 1 View

What statistical test should I use if I want to compare values of temperature taken at aural, forehead and inner wrist ?

I basically want to see if there is a difference between these sites. Especially between the forehead and inner wrist as many patients are not very happy with temperature reading taken at the...

05 October 2020 3,151 12 View

Need weather and metrological data for forecasting or predicting the agriculture in Saudi Arabia?

Need weather and metrological data for forecasting or predicting agriculture in Saudi Arabia? This is for the purpose of one of the project by my fellow researchers. Kindly share the same.

11 August 2020 8,163 4 View

What are the high impact journals available in sustainability and sustainable development domain?

24 June 2020 3,979 11 View

What are the COVID-19 papers retracted from journals?is it clinical, treatment, diagnosis or AI based prediction? Why?

What are the COVID-19 papers retracted from journals? is it related to clinical, treatment, diagnosis or AI-based prediction? Why

07 June 2020 7,301 3 View

Any suggestions for SCOPUS Indexed journal for social science field, specifically for education?

I'm looking for any SCOPUS indexed journal to send my article. Social science specifically in education field with minimum waiting period for the acceptance. I did contacted some journal editors...

20 April 2020 8,242 1 View

Hydroxychlorine was proposed as drug for corona viral disease (COVID-19). Is it good for treatment?

It might induce immunosuppression.

03 April 2020 3,740 4 View

Quantitative & Qualitative perceptive. (mix mode)?

Dear All, My research topic is socio-economic impact of employment of Filipinos Maids in Singapore. My research will be both Quantitative & Qualitative perceptive. (mix mode) My question:...

19 October 2019 5,959 8 View

How can we determine the sample size from an unknown population?

Please provide example too, im new ...

09 April 2019 5,724 4 View

Transformed colonies with GFP showed Faded Fluorescence ?

Transformed Yeast colonies with a Plasmid having GFP, showed faded Fluorescence. It was light yellowish in colour rather than the usual Green Colour . What could be the reason ? The Microscope was...

28 December 2018 7,516 4 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

How does the gut microbiome affect the fibrotic process within the liver?

and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?

02 March 2021 557 2 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View

Rajaram Krishnasamy

RAW 264.7 Cell Line (murine Macrophage)

Bokang Cui

L02 is normal liver cell line

Aruljothi Subramaniam

Guys thanks for ur replies.......

Murat Tosun

L02 is very useful

Shaista Jabeen

Thanks this information is very useful for my studies. What about renal cell line which is normal cell line for RCC.

From

shaista jabeen