20 July 2011 5 8K Report

Can anyone recommend which normal cell line would be appropriate for comparison study for any drug treatment in compared with liver cancer cell lines?

More Aruljothi Subramaniam's questions See All
Why I'm unable to observe insert sequence bands in Colony PCR?

Hi, I have grown BL21 cells with the pET28a that carries my insert sequence. It is growing on the selection plate but I can't observe any insert sequence bands when I do colony PCR. Is there any...

15 July 2024 5,135 5 View

Which modeling software and force field suitable for miRNA-mRNA molecular simulation?

Hello everyone, I am currently working on miRNA- mRNA interaction study. Further, I would like to do MD simulations for the miRNA-mRNA models. For the Simulation, I have modelled this complex...

24 June 2024 3,507 2 View

Please help me identify what is wrong with my cell culture. What type of contamination is this?

I have harvested human dermal fibroblast cells and subcultured into T25 flasks using fibroblast expansion medium that contant 10% LSGS and 1% anti-anti. When observe the cells a day after...

16 May 2024 8,239 0 View

How to induce substitution mutation to a 3D Protein in silico?

Hi, I am working on protein-protein interaction studies, specifically on antibody-antigen interaction. I would like to observe the changes in interaction if there's mutation occurs in the protein....

04 October 2023 8,796 3 View

How to design a reverse primer for poly A tailed microRNA?

Hello, I am working on microRNA expression studies on Regeneration. I isolated RNA from my sample and converted it into cDNA using Poly A polymerase and Reverse transcriptase enzyme. Previously I...

18 August 2023 3,898 0 View

I am shocked to see that the co-author of my journal article has been changed?

How is this possible on ResearchGate?????

20 July 2023 4,448 3 View

How to avoid ligand and receptor chain from merging into one chain after molecular docking?

Hi all, I've performed molecular docking using ClusPro. One of the chain from the ligand merged to a chain from the receptor when I wanted to study the protein-protein interaction using iCn3D....

02 July 2023 3,720 4 View

How to identify the binding sites of the antigen using GROMACS?

Hi, I am working on protein-protein interaction studies, specifically on antibody-antigen interaction.

19 June 2023 9,853 5 View

Hello Science community, I would like to ask whether tpp tubespin can be autoclaved especially the filter cap??

please help anyone have suggestion?

03 August 2022 1,038 0 View

Anyone can help to solve the Error in autodock running?

Hi, I am facing following issue at the time of running of Autodock 4.2 even after completing the all the steps properly. Its common for even different protein which were tried to dock with...

18 May 2022 8,950 0 View

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Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Radiogenomics Cancer Research Challenges?

what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...

03 August 2024 5,828 4 View

Tissue homogenization in mouse with FastPrep-24™ 5G - is the D matrix system useful?

I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...

31 July 2024 9,186 0 View

For a case-control study for Breast Cancer incidents in a particular County in USA, what data can be used as Control? E.g. colorectal cancer?

The County health department is providing Breast cancer incident data. If colorectal cancer cases could be used as control, then we can get respective data from health department as well.

22 July 2024 9,954 4 View

About Machine Learning for Automated Diagnosis of Skin Cancer with Dermoscopy Images ?

Machine Learning for Automated Diagnosis of Skin Cancer with Dermoscopy Images

21 July 2024 5,471 0 View

Fixation Problem with cancer cells?

Hello everyone, I work with polyacrylamide hydrogels, which I fictionalise with sulfo-sanpa and collagen I to then plate lung cancer cells. The problem is that I lose a significant amount of...

19 July 2024 3,906 0 View

Does anyone notice the tumor regression impact of Hepa1-6 cells in HCC orthotopic mice model?

To establish an orthotopic HCC model, C57BL/6 mice (male, 6-8 weeks) were orthotopically implanted with 3x106 Hepa1-6 cells (Over-expressed with a human gene) in 20uL Matrigel into the left lobe...

18 July 2024 9,177 2 View

How to prepare bacterial conditioned media to study the effect of bacterial secretome?

I would like to investigate the effect of bacterial secretome on cancer cells. How do you prepare conditioned media from bacteria?? conditions- time points - media I am looking for the best way...

17 July 2024 3,785 9 View

Contaminant cells in pancreatic cancer patient derived organoids?

Hello everyone, I am cultivating patient derived organoids from pancreatic cancer patients and I saw at the bottom of my domes some "strange" cells other than my usual organoids. I know I should...

15 July 2024 762 3 View

Oncologist at Visakhapatnam interested to collaborate?

Calling all medical professionals and researchers! I'm seeking recommendations for an oncologist in Visakhapatnam who can collaborate on my PhD thesis. My research focuses on stage 1 and 2 gastric...

14 July 2024 936 0 View

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