NFkB indution with TNFa in cell lines (Jurkat)?

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A good reliable cellular hypoxia assay?

I'm going to expose cells to hypoxic conditions, in order to establish the function of a synthetic promoter containing HRE's. For control sake, I want to see if cells actually developed hypoxic...

31 December 2016 6,919 4 View

IL-2 toxicity in murine model?

What are the toxic levels in I.P or I.M injections for mice? I know there is a range, and I think I even read a paper stating it plainly, but I seem to misplace it, and can't find it. Does any one...

11 December 2016 8,410 1 View

Kidney and liver function blood test in mice?

What are the parameters most required? For liver is there anything more than AP, ALT and AST? And for kidney anything more than Creatinine and Uric acid?

09 October 2016 2,363 1 View

Can I use a sandwich ELISA ab for WB? If so, which one? for the capture or the detection of biotin conjugated?

Read 3 answers by scientists with 3 recommendations from their colleagues to the question asked by Aner Ottolenghi on Oct 13, 2016

09 October 2016 8,931 4 View

MHC induction with Interferon gamma (IFNg)? What are the recommended amounts, and how long does it take?

Read 5 answers by scientists to the question asked by Aner Ottolenghi on Sep 20, 2016

08 September 2016 5,432 5 View

Culturing CTLL cells with IL-2?

So my supervisor insists that you can culture CTLLs wih IL-2, instead of the ATCC recommended - Con A an T-STIM. It makes sense, after all propogation of these cells with IL-2 is the ED50 test for...

08 September 2016 10,045 3 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

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09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

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How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

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Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

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The Curse of Evolution and Complexity?

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How to isolate lymphocytes from mouse spleen?

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How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

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Charles Chang

30ng/ml

treatment duration depends on your analysis method

Jordan R. Yaron

I've detected robust activation using an NFkB reporter at 10 ng/mL over 18-24 hours.

Aner Ottolenghi

What kind of reporter are you using? I thought about using ICAM-1 antibodies for FACS.

I am using a Hek293-based reporter with 5x NFkB binding sites ahead of a secreted alkaline phosphatase coding sequence.

Cool, thanks.