What are the recommended quantities and duration until induction?
30ng/ml
treatment duration depends on your analysis method
I've detected robust activation using an NFkB reporter at 10 ng/mL over 18-24 hours.
What kind of reporter are you using? I thought about using ICAM-1 antibodies for FACS.
I am using a Hek293-based reporter with 5x NFkB binding sites ahead of a secreted alkaline phosphatase coding sequence.
Cool, thanks.
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