It depends upon cell-type and the source of the IFN-gamma. In general, 10 ng/ml of recombinant IFN-gamma can appreciably induce certain MHC genes within 6-12 h. However, the time needs to be optimized with each batch of the IFN-gamma.
Hi Aner,
I agree with Dr. Choubey that it varies on cell type. Most of our experience is with human culture systems where we have generally used 100 Units/mL or more for periods between 24-48 hours. If possible try to find out the activity of the cytokine that you are using since it varies greatly from manufacturer to manufacturer which makes it difficult to interpret ng/ mL as a unit of measurement.
Good luck!
Hello everyone, I'm also trying stimulate MHC with interferon gamma.
I'm using PBMC and my interferon is 2x107 U/mg.
what concentration of cells per mL do I need to stimulate with 100 U of interferon ( in my case 0,05 ul of interferon)
Can you help me?
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