Aner Ottolenghi

7 Questions 12 Answers 0 Followers

Questions related from Aner Ottolenghi

A good reliable cellular hypoxia assay?

I'm going to expose cells to hypoxic conditions, in order to establish the function of a synthetic promoter containing HRE's. For control sake, I want to see if cells actually developed hypoxic...

01 January 2017 6,880 4 View

IL-2 toxicity in murine model?

What are the toxic levels in I.P or I.M injections for mice? I know there is a range, and I think I even read a paper stating it plainly, but I seem to misplace it, and can't find it. Does any one...

12 December 2016 8,373 1 View

Can I use a sandwich ELISA ab for WB? If so, which one? for the capture or the detection of biotin conjugated?

Read 3 answers by scientists with 3 recommendations from their colleagues to the question asked by Aner Ottolenghi on Oct 13, 2016

10 October 2016 8,898 4 View

Kidney and liver function blood test in mice?

What are the parameters most required? For liver is there anything more than AP, ALT and AST? And for kidney anything more than Creatinine and Uric acid?

10 October 2016 2,324 1 View

NFkB indution with TNFa in cell lines (Jurkat)?

What are the recommended quantities and duration until induction?

09 September 2016 7,519 5 View

MHC induction with Interferon gamma (IFNg)? What are the recommended amounts, and how long does it take?

Read 5 answers by scientists to the question asked by Aner Ottolenghi on Sep 20, 2016

09 September 2016 5,385 5 View

Culturing CTLL cells with IL-2?

So my supervisor insists that you can culture CTLLs wih IL-2, instead of the ATCC recommended - Con A an T-STIM. It makes sense, after all propogation of these cells with IL-2 is the ED50 test for...

09 September 2016 10,016 3 View