Dear Omur,

Merhaba,

It is not clear what do you mean by quality control, or what qualities you are after? Do you mean percentage or ratio of cells differentiating to become neurons?

In our lab we are producing neurons from human iPS cells. We use a number of neuronal markers, but I personally prefer electrophysiological testing to be sure. I consider cells as neurons only if they are able to display extracellular spiking activity or fire action potentials (spontaneous or evoked), or if they have fast activating and inactivating voltage-gated sodium currents. If not then I consider them as immature neurons or neuronal progenitors.

best wishes,

Refik

Dear Refik,

The tests that are going to make us sure of ips cells which differentiates into neurons.

Thanks a lot.

Dear Omur,

There are lots of publications that rely on gene expression studies to determine differentiation into a neuron, and you may choose that pathway. However, as an electrophysiologist my criteria to define a neuron is different than that of many cell biologists (see my comment above). From my experimental work I am convinced that the majority of cells previously described as neurons are actually immature neuronal progenitors, without the ability to fire action potentials.

best wishes (hoscakal) Refik

Conference Paper Extensive heterogeneous dye-coupling among cells derived fro...

Hello

# Immunostaining

 # reverse transcription-polymerase chain reaction (RT-PCR) analysis

# electrophysiological testing

# microRNA (miRNA)

http://www.stemcellres.com/content/4/3/73

http://www.sciencedirect.com/science/article/pii/S1873506111001619

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2895909/

http://link.springer.com/article/10.1007%2Fs00441-014-1981-y

http://onlinelibrary.wiley.com/doi/10.1111/cbdd.12485/abstract