All the experimental results need to be presented with statistical analysis. In case of adsorption isotherm and kinetic study, what does I include?

What are the things should I include under statistical analysis.

19 November 2020 5,098 2 View

How to clone a promoter region of a gene within pBI101.2 vector for GUS assay?

I am cloning about 1.5kb promoter of a gene within pBI101.2 vector for GUS assay. During the primer designing, I am facing a problem. Should I include some portion(say about +20 to +30 from the...

10 June 2019 6,950 2 View

What are the methods to follow if I want to study cross talk between molecular pathways?

If I want to study the cross talks between pathways involving more than 20-30 genes, what are the methods I should follow? qRT PCR and then validation by Western Blot or Immunohistochemistry is...

19 June 2018 7,134 2 View

No title

19 June 2018 6,121 3 View

How to plot in-plane vibration modes of square plate using MATLAB. The plot looks like a distrorted X-Y plane grid ?

I have the natural frequencies and the related displacement equations in x, y directions for in-plane vibration. Every point in the plate has a displacement in x (denoted by u(x,y) ) and y...

30 October 2017 304 11 View

How to check purity of mono-/bis-/metal complex-azo dyes using UV-Vis Spectroscopy?

How to check purity of mono-/bis-/metal complex-azo dyes using UV-Vis Spectroscopy?

22 October 2017 3,767 6 View

Given a bare-Lagrangian for a renormalizable, interacting system, is the renormaized version unique? If yes, then which ?

It is well-known that several renormalized versions of a given theory may emerge depending upon the chosen regularization method, choice of subtraction scale/scheme,...

09 March 2017 8,192 40 View

Perovskites are a class of compounda, than how can we identify that which material is suited for harvesting solar energy?

26 February 2017 4,620 3 View

Between ER and SQ grade reagents of Qualigens (Thermo fisher) which one is analytical grade [AR] reagent ?

23 November 2016 716 7 View

Why the ligand structure changes during molecular dynamics simulation?

I did MD simulation for 10 ns. the ligand was inside the simulation box upto 10ns. only in the 3rd ns the structure was ok. in all other time one of its double bond was missing. many times the OH...

23 December 2015 9,118 6 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...

21 February 2021 5,379 2 View

Can anyone help or comment on solubilising chlorobutanol in aqueous injections in minimum time possible?

Please suggest solubilisation of chlorobutanol in injections. Tried pH and high temperature. But the issue is of assay drop. If anyone can suggest any solution. Its a commonly used excipient for...

21 February 2021 7,397 1 View

Did anyone perform CD107a degranulation assay without the use of inhibitors like monensin?

I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...

21 February 2021 6,260 3 View