Omar N. Issa

3 Questions 1 Answers 0 Followers

Questions related from Omar N. Issa

Does anyone have an idea whether DCFH-DA is oxidized by H2O2 only or by other ROS?

I have exposed zebrtafish embryos to 3 toxicants of which 2 of the toxicants were individually added while the third one was a mixture of the first two. When I measured the intensity of the plate,...

07 December 2018 5,467 3 View

Can anyone refer to a cytosolic/nuclear protein fractionation protocol ?

I hope this finds you all well, I will be soob have to extract the nuclear protein Nrf2 from zebrafish embryos homogenate. I have to saperate the cytosolic protein from nuclear protein, which is...

15 August 2018 6,135 1 View

Im practicing microinjection using phen red in minn oil, every time I inject, the beads burst& cant get their size on the micrometer, any clues why?

I am practicing microinjection now using phenol red and trying to measure the bead size on the micrometer. Everytime I inject the phenol red into the minneral oil, the beads immedietly burst and I...

07 April 2018 421 0 View