Are there any metastatic breast cancer cell lines derived from Swiss or B6 mice?
T/SA is a good model to study mestastatic breast cancer
It is easy to produce linearized ends with sticky ends using restriction enzymes and these sticky ends are readily ligated by DNA ligase enzyme alone without the need for DNA end-processing. How...
03 April 2015 3,210 4 View
I can understand multiple individuals having same first AND last name but it is definitely a better way to search, in my experience, than last name ONLY or last name AND initial of the first name....
11 December 2014 3,533 2 View
Both ResearchGate and researchers should inform us in reply to this query regarding take-down notice(s) issued by any journal/publisher so we all sensitive and not create repeated headache to our...
10 November 2014 7,393 0 View
Can prostate, salivary, or other glandular tissues express/secrete milk proteins under experimental conditions in any model organism? The ability of breast epithelial tissue to express/secrete...
09 October 2014 8,634 4 View
It appears to me that most plasmid DNA entering a human cell gets degraded (perhaps rapidly but not sure about the rate) and only a small portion find their way into the nucleus. What exactly...
09 October 2014 5,747 2 View
Has anyone looked at this? I would like to know the probability of each linear or circular plasmid (10kb) transfected into a human epithelial cell to survive and express the transgene. Do majority...
05 June 2014 9,133 2 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View
what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...
03 August 2024 5,828 4 View