Has anyone looked at this? I would like to know the probability of each linear or circular plasmid (10kb) transfected into a human epithelial cell to survive and express the transgene. Do majority of them get degraded? Please direct me to studies that may give me some clues.
Hi Nagarajan,
I don't have an exact reference for you, but when I was working on my PhD (back in 2004) the assumption was that the vast minority (
Hi Jonathan
I appreciate your response. Its surprising to me that there aren't many quality studies on the kinetics of plasmid DNA degradation in the literature. Your answer was helpful and did iterate what I thought was happening to these plasmids in the cytoplasm. I am setting up experiments to get some answers myself and will post my findings here for everyone's benefit. Thanks again.
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