Hello everyone,
I was told that it is possible to measure NADPH level using autofluorescence: excitation 350, emission 450/50. Did anyone try the method? What are limitation, knowhows, specificity and signal level?
Could you please recommend how to choose a proper equivalent pixel size (i.e. to which size of image space relative to PSF should correspond a single pixel on camera matrix) so that my imaging...
11 December 2012 8,800 1 View
I cannot find an article, that describes a successful use of metric other than SNR/MSE to characterize deblurring of fluorescent images. Could you please recommend me one, preferably using no...
10 November 2012 4,377 1 View
My data is usually represented as 3D data (stack of several 2D fluorescent images, each with constant displacement along optical axis from the other), common voxel size is 100*100*300 nanometer....
10 November 2012 8,730 0 View
I found an article (see link attached) which describes a method how to decrease oversegmentation while doing watershed. The method proposed really works for 8bit images, but I cannot implement it...
07 August 2012 8,312 6 View
Dear all! There are several approaches to create 3D (??not always clear) map of refractive indexes of object using either standard phase contrast, or DIC, or common brightfield imaging. The most...
11 December 2011 559 0 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)
05 August 2024 9,537 2 View
We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...
31 July 2024 4,510 3 View
Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...
29 July 2024 8,624 1 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
Hello; I am conducting a CFD study on two-phase flow in a separator, utilizing refrigerant as the working fluid. For this study, I have implemented pressure inlet and pressure outlet boundary...
25 July 2024 2,309 5 View
Hi everyone, I am working on a curved domain in which a ship is situated in the middle (geometry is given below). In my understanding the general fluid flow is parallel to the x axis from inlet to...
25 July 2024 9,058 4 View
Hello, I'm conducting flow cytometry experiments using the BD Cell Viability Kit and I've noticed that the TO solution ends much faster than the PI solution. I store the solutions according to...
22 July 2024 2,257 0 View
I am giving starvation stress to the soil amoeba (Dictyostelium discoideum) cells in my study groups and using NAC as a negative control. I am using DHE for ROS measurement.
20 July 2024 6,354 0 View
In terms of CFD, we often analyze the stability of the error using Von-Neumann analysis, especially for FDM based problems. Should we follow the same approach for a compressible fluid flow using FVM ?
13 July 2024 6,296 5 View