Choosing a proper sampling aperture/equivalent pixel size

Could you please recommend how to choose a proper equivalent pixel size (i.e. to which size of image space relative to PSF should correspond a single pixel on camera matrix) so that my imaging...

11 December 2012 8,735 1 View

Quality assessment in fluorescent microscopy: testing deblurring

I cannot find an article, that describes a successful use of metric other than SNR/MSE to characterize deblurring of fluorescent images. Could you please recommend me one, preferably using no...

10 November 2012 4,318 1 View

How to assess quality of extension to multidimensional data?

My data is usually represented as 3D data (stack of several 2D fluorescent images, each with constant displacement along optical axis from the other), common voxel size is 100*100*300 nanometer....

10 November 2012 8,669 0 View

Practical approach to Weber's Law (image quintization)

I found an article (see link attached) which describes a method how to decrease oversegmentation while doing watershed. The method proposed really works for 8bit images, but I cannot implement it...

07 August 2012 8,262 6 View

Correction for specimen-induced aberrations in 3D fluorescent microscopy

Dear all! There are several approaches to create 3D (??not always clear) map of refractive indexes of object using either standard phase contrast, or DIC, or common brightfield imaging. The most...

11 December 2011 482 0 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Why do the air flow results vary totally when adding scalar in CFD ?

Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...

25 February 2021 9,602 4 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

How to calculate time-averaged fields from Ansys CFX solver?

I would appreciate any comments on the following problem. I need to calculate mean fields (e.g. pressure, velocity and vorticity), for which I activate Trn Stats tab in CFX-Pre. However, what I...

21 February 2021 8,548 7 View

Which software is best to analyse multi-phase fluid flows ?

Hello everyone, hope all is good. I want to analyse multi-phase fluid flow through pipe, which software is best to do so ?

21 February 2021 4,230 8 View

Can anyone help with an Accurate Diffuser Expansion Loss formula for rectangular shaped diffusers in turbulent flow regime?

I have tried Idelchik's Handbook of Hydraulic Resistance 4th Edition (2007 page 300) formulas and across some range the formulas are reported valid for, the law of conservation of energy is...

20 February 2021 2,504 1 View

Oil red O protocol for flow cytometry?

18 February 2021 4,214 3 View

How to read a flow cytometry graph detailing cell cycle?

Hello everyone, When determining cells in different stages of the cell cycle, cells are stain for Brdu and Propidium Iodide. I understand how PI can be used to discriminate between G1 and S/G2...

18 February 2021 3,920 3 View