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Questions related from Sergey Tauger
Could you please recommend how to choose a proper equivalent pixel size (i.e. to which size of image space relative to PSF should correspond a single pixel on camera matrix) so that my imaging...
12 December 2012 8,772 1 View
My data is usually represented as 3D data (stack of several 2D fluorescent images, each with constant displacement along optical axis from the other), common voxel size is 100*100*300 nanometer....
11 November 2012 8,706 0 View
I cannot find an article, that describes a successful use of metric other than SNR/MSE to characterize deblurring of fluorescent images. Could you please recommend me one, preferably using no...
11 November 2012 4,347 1 View
I found an article (see link attached) which describes a method how to decrease oversegmentation while doing watershed. The method proposed really works for 8bit images, but I cannot implement it...
08 August 2012 8,291 6 View
Hello everyone, I was told that it is possible to measure NADPH level using autofluorescence: excitation 350, emission 450/50. Did anyone try the method? What are limitation, knowhows,...
03 March 2012 5,016 0 View
Dear all! There are several approaches to create 3D (??not always clear) map of refractive indexes of object using either standard phase contrast, or DIC, or common brightfield imaging. The most...
12 December 2011 534 0 View
